You’ll notice that Image 2 now shows up in the Image 1 window, but is greyscale. Select the green channel and select all (press the CTRL-A key combo, or Select | All from the file menu), copy (press CTRL-C or Edit | Copy), select Image 1, select the Red channel and paste (press CTRL-V or Edit | Paste). Goto Image 2’s Green channel (select image 2, click Window | Channels, highlight the channel labeled Green). image shows up in more than one) you may have to manipulate the image prior to doing the following).Ĭopy the Green Channel from Image 2 and Paste it into the Red Channel of Image One If you images have multiple channels used (i.e. From what we’ve seen most of the image acqusition systems save the image with only 1 channel, but they could be Red or Blue. This is completely dependent on the source of the images. I will choose image 1 to be Green, Image 2 to be Red and 3 to be blue.Īll three of these images only have one channel, the green channel (thus them being green). labeled 1 - 3 for easy identification, but you can combine and duplicate this process on any number of images to fit your purpose.įor this example I will leave the images in RGB format. This is not an explanation of or advocate for collocation analysis, simply how to create the images.įor the sake of simplicity, I will work with only three images in fig1. If you have an older version of Photoshop, the below is still possible, but location and name of tools/windows may be in different. ![]() This tutorial will go over a few ways to accomplish this in Photoshop CS3 using the Channel feature. However, a frequent request is how to overlay/merge similar tissue images with different fluorescent stains. Photoshop and other programs simplify this process by using the layers concept. The structure of the commands can be quickly learned with a short trip to the manual and following the example.A common practice with images is to merge or overlay them to form one image. But notice how a single interface is able to handle all the different elements homogeneously with just a few commands: fill, join, and typeset. In fact, similar output can be obtained using other LaTeX packages and commands. ![]() Note that we can easily go through the left and right margin just by using the offsets. Being a text object it is not affected by the floating mechanism, another very useful plus. The last step is just a command to put the entire assembly at the current insertion point. For the table, three joins are needed, for the main figure four: the complete table, already assembled, the figure caption, the inserted figure and the side text. The second step is to assemble the xcoffins two by two. So the first task is to fill the xcoffins with your content, including its format: fonts, colors, figures, text, tables, etc. In this sense, except for the lack of a GUI, it works in the same way that a layout design program. Xcoffins are just boxes with added special points (handles) that allow them to be attached, one to another, precisely, and eventually add X Y offsets.Īn important feature of the xcoffins tool is the separation between content and layout. It consists of the table itself plus three labels, on the top, bottom and left sides, the last one rotated 90 degrees. The table itself is, as always, the most complex element. ![]() ![]() So I envisioned a scenario that included two additional, and more demanding elements: a table: a LaTeX table mimic of the inset shape of the question, and a larger explanatory lateral side text, with the whole assembly crossing both margins of the text area. For two figures, the problem was deftly solved by the previous answer (Werner's). I wanted to show the power and simplicity of xcoffins in solving the kind of problems the question posed: how to put different elements together using their relative positions.
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